A consensus in Plasma Medicine procedures. New publication in Nature Protocols

ERC APACHE project just released its last work "A protocol in plasma-medicine: evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures" in Nature Protocols, a journal with high standards and influence in the standardization of science.

The publication involves the use of cold atmospheric plasma jets for biological and medical applications, finding particular application in cancer, although it could be also applied to other areas such as wound healing or tissue regeneration. The protocol states as a consensus for the field of Plasma Medicine to apply plasma treatment in the same experimental conditions (different plasma devices & treatment conditions, different culture media, different well size, etc.), claiming to overcome the current limitations regarding the comparison & interpretation of results among laboratories/research groups. Another essential aspect tackled by this protocol is the upscaling of the plasma treatments to different cell culture sizes which allow to perform biological assays beyond cytotoxicity, such as molecular assays, in a reliable manner.

The protocol focuses either on direct exposure to Cold Plasma and/or indirect exposure through Plasma Activated Liquids and investigate their effects on cell viability, its relationship to the reactive species generated in the culture media, and – very importantly - allowing to investigate also the molecular mechanisms affected by the treatment.

We invite you to take a look to this outstanding publication: https://www.nature.com/articles/s41596-021-00521-5

Tornin, J., Labay, C., Tampieri, F. et al. Evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures. Nature Protocols volume 16, pages2826–2850 (2021). https://doi.org/10.1038/s41596-021-00521-5